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LightLab Imaging
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TAIWAN HIPOINT
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Image Search Results
Journal: Applied and Environmental Microbiology
Article Title: The Blue-Light Photoreceptor Sfwc-1 Gene Regulates the Phototropic Response and Fruiting-Body Development in the Homothallic Ascomycete Sordaria fimicola
doi: 10.1128/AEM.02206-18
Figure Lengend Snippet: S. fimicola SfWC-1-GFP preferentially accumulated in the nuclei of vegetative hyphae under blue-light illumination and darkness conditions. (A) The localization of SfWC-1-GFP expressed in the WT was restricted in nuclei under darkness or 15- and 60-min blue-light treatments. (B) The WT strain containing H2B-mCherry and SfWC-1-GFP was grown under darkness or 15- and 60-min light induction conditions. H2B-mCherry fluorescence signal was used to monitor the location of nuclei. Live cell images of mycelial samples were collected before (DD) and after blue-light treatments for 15 and 60 min (DL15 and DL60). The LED blue-light intensity was 20 to 35 μmol/(m2 · s). White arrows indicate nuclear localization. The fluorescence of live cells was examined by using a spinning-disk confocal imaging system. All images were acquired under similar conditions, including illumination exposure setting and contrast enhancement.
Article Snippet: The light sources used in the experiment were fluorescent white light of 400 to 700 nm (30 to 40 μmol/[m 2 · s]; Goodly Electric, Taiwan) and a light-emitting
Techniques: Fluorescence, Imaging